Cloning, sequencing and mapping of a manganese superoxide dismutase gene of the nematode Caenorhabditis elegans.
نویسندگان
چکیده
We have cloned, sequenced and mapped a gene (sod-2) encoding manganese superoxide dismutase [EC 1.15.1.1] from the nematode Caenorhabditis elegans. The sod-2 was mapped to chromosome I by hybridization with a YAC polytene filter. The protein-coding region spans 1129 base pairs including 4 introns and encodes a protein of 221 amino acids (aa) (M(r) = 24536) of which the first 24 aa are the presumed mitochondorial-targeting signal peptide. The gene sequence of sod-2 was slightly different from an isoform, sod-3.
منابع مشابه
Cloning, expression, and characterization of two manganese superoxide dismutases from Caenorhabditis elegans.
Two genes encoding manganese superoxide dismutase (sod-2 and sod-3) have been identified in the nematode Caenorhabditis elegans. Each gene is composed of five exons, and intron positions are identical; however, intron sizes and sequences are not the same. The predicted protein sequences are 86.3% homologous (91.8% conservative), and the cDNAs are only 75.2% homologous. Both deduced protein sequ...
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ورودعنوان ژورنال:
- DNA research : an international journal for rapid publication of reports on genes and genomes
دوره 3 3 شماره
صفحات -
تاریخ انتشار 1996